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为什么核移植时常用去核的MⅡ中期卵母细胞?1问题[网友AX]在人教版高中生物教科书《现代生物科技专题》P.48的体细胞核移植过程流程图中,为什么用的是处于MⅡ中期次级卵母细胞作为受体细胞?教科书中说,由于MⅡ期卵母细胞核位置靠近第一极体,用微型吸管可以一并吸出细胞核与第一极体。中期不是核消失了吗?吸的时候极体和卵母细胞还在一个细胞中吗?图上的一个小红点是什么?2讨论李雪峰[华南师范大学生命科学学院(510631)]细胞核移植常用受体细胞是处于MⅡ中期的卵母细胞,这是由于第一极体与中期染色体位置很近,可以通过去除极体附近的部分细胞质的方法把中期染色体去掉。正如您提到的,这个时候没有细胞核只有中期染色体,一般将去除中期染色体的过程称为去核,
Why nuclear transplantation commonly used enucleated M Ⅱ medium-term oocyte? 1 Question [User AX] in the PEP high school biology textbook “modern biotechnology topics” P.48 somatic cell nuclear transfer process flow chart, why is the use of MII secondary secondary oocytes as receptor cells? Textbook said that as M Ⅱ oocyte nuclear position close to the first polar body, with a micropipette can suck out the nucleus and the first polar body together. Mid-term is not nuclear disappear? Pole and oocyte when the suction is still in a cell? A little red dot on the map is what? 2 Discussion Li Xuefeng [South China Normal University College of Life Sciences (510631)] Commonly used in nuclear transfer Recipient cells are mid-M Ⅱ oocytes, which is due to the first polar body and metaphase chromosome close position, you can remove part of the cytoplasm near the polar body method to remove metaphase chromosomes. As you mentioned, at this time there is no metaphase chromosome in the nucleus, the process of removing metaphase chromosomes is usually called enucleation,