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分别用20、50、100mg/(kg体重)3个剂量的达氟沙星(Danofloxacin)水溶液连续口灌施氏鲟(Acipenserscherensckii)20d,并于给药后第5、10、15、20天用试剂盒测定肝脏SOD、CAT、GOT和GPT的活性及MDA和NO的含量。施氏鲟体重75～95g。结果表明,短时间内给药后(5天),100mg/(kg体重)组SOD活性与对照组相比提高显著(P<0 01)。随着实验时间的延长(第10、15天),与对照组相比各实验组SOD酶活性均保持在较高活性(P<0 01)。至第20天,各实验组SOD活性呈下降趋势,但仍明显高于对照组。给药后第5天时,各实验组CAT活性与对照组之间无差异;至第10、15天时,50、100mg/(kg体重)组均极显著高于对照组;给药后第20天,仅100mg/(kg体重)组显著低于对照组。实验第5、10、15、20天时,各实验组与对照组之间GPT和GOT活性均没有明显的差异性。仅在相同实验组内随着时间的改变有些变化。与对照组相比,在实验第5、10、15、20天各剂量组MDA含量均无显著的变化。20、50mg/(kg体重)组在第5、10、15天时有所降低,但至第20天时均恢复到对照组的水平;100mg/(kg体重)组至第15天降至最低,但实验结束时反而明显高于对照及其他实验组。从实验结果看,在实验第5、10、15、20天,各实验组施氏鲟肝脏NO含量与对照组相比较没有明显的变化。结果表明,达氟沙
Acipenserscnnsnskii were consecutively dosed with 20, 50 and 100 mg / (kg body weight) of 3 doses of Danofloxacin aqueous solution for 20 days. The activity of SOD, CAT, GOT and GPT and the content of MDA and NO in liver were measured by kit. Sturgeon weight 75 ~ 95g. The results showed that the SOD activity in 100 mg / (kg body weight) group increased significantly (P <0.01) compared with the control group after short-time administration (5 days). With the extension of experimental time (days 10 and 15), the activity of SOD in each experimental group remained higher than that in the control group (P <0.01). By the 20th day, the SOD activity in each experimental group showed a downward trend, but still significantly higher than the control group. On the 5th and 5th days after administration, there was no difference between the CAT activity in each experimental group and the control group. On the 10th and 15th days, the levels of CAT and CAT in the 50 and 100 mg / (kg body weight) groups were significantly higher than those in the control group , Only 100mg / (kg body weight) group was significantly lower than the control group. At the 5th, 10th, 15th and 20th day of the experiment, there was no significant difference in GPT and GOT activity between experimental groups and control groups. Only some changes in the same experimental group over time. Compared with the control group, there was no significant change in the MDA content of each dose group on the 5th, 10th, 15th and 20th day of the experiment. 20, 50mg / (kg body weight) group decreased on the 5th, 10th and 15th days, but recovered to the control group on the 20th day; the group of 100mg / (kg body weight) reduced to the lowest on the 15th day At the end of the experiment, however, it was significantly higher than the control and other experimental groups. From the experimental results, on the 5th, 10th, 15th and 20th day of the experiment, there was no obvious change in the NO content in the liver of the experimental group compared with the control group. The results show that daflusa