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目的研究不同浓度锌对成骨细胞骨保护素基因表达和细胞增殖的影响。方法取小鼠的成骨细胞系MC3T3-E1培养并随机分为4组,A组加入10μmol/L硫酸锌;B组加入50μmol/L硫酸锌;C组加入200μmol/L硫酸锌;D组作为空白对照。培养48h后提取细胞RNA,RT-PCR分析成骨细胞骨保护素(OPG)mRNA表达,Elisa检测细胞培养上清中骨保护素表达水平。MTT法测定成骨细胞增殖率。结果 A组、B组、C组与D组细胞骨保护素基因表达比值分别为0.461±0.051、1.068±0.123、0.244±0.044、0.548±0.089,A组与D组差异不明显,B组高于D组(P<0.01),C组低于D组(P<0.01)。Elisa结果与RT-PCR相同。细胞增殖率之间差异有显著性意义,B组高于D组(P<0.01),C组低于D组(P<0.01)。结论 50μmol/L的锌促进成骨细胞骨保护素基因表达和细胞增殖,200μmol/L的锌则抑制成骨细胞骨保护素基因表达和细胞增殖。而10μmol/L的锌对成骨细胞骨保护素基因表达和细胞增殖的影响不大。
Objective To study the effect of different concentrations of zinc on osteoblast osteoprotegerin gene expression and cell proliferation. Methods The mouse osteoblastic cell line MC3T3-E1 was cultured and randomly divided into 4 groups. Group A received 10μmol / L zinc sulfate; Group B received 50μmol / L zinc sulfate; Group C added 200μmol / L zinc sulfate; Group D received Blank control. After cultured for 48h, the cell RNA was extracted. The expression of osteoprotegerin (OPG) mRNA was detected by RT-PCR. The expression of osteoprotegerin in cell culture supernatant was detected by Elisa. MTT assay osteoblast proliferation rate. Results The ratios of osteoprotegerin gene expression in group A, group B, group C and group D were 0.461 ± 0.051, 1.068 ± 0.123, 0.244 ± 0.044 and 0.548 ± 0.089, respectively. The difference between group A and group D was insignificant, while group B was higher than D group (P <0.01), C group was lower than D group (P <0.01). Elisa results are identical to RT-PCR. There was a significant difference between the two groups (P <0.01), group B (P <0.01) and group C (P <0.01). Conclusion 50μmol / L zinc can promote osteoblast osteoprotegerin gene expression and cell proliferation, while 200μmol / L zinc inhibits osteoblast osteoprotegerin gene expression and cell proliferation. However, 10μmol / L zinc had little effect on osteoblast osteoprotegerin gene expression and cell proliferation.