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本研究以普通小麦品种'中国春'染色体组DNA为封阻,用生物素(biotin-16-d UTP)标记的大麦染色体组DNA作为探针,通过基因组原位杂交法解析了来自杂交组合CS×(CS+Betzes 2H)杂种后代X99-13的遗传组成,此材料含有42条染色体,其中1条大麦染色体,2条小麦-大麦易位染色体和39条小麦染色体,鉴定为小麦-大麦代换易位系.以小麦第二部分同源群短臂探针psr131进行RFLP分析,结果表明此代换易位系是涉及小麦染色体2B和大麦染色体2H的代换易位.为进一步选育小麦-大麦2H纯合易位系及利用其上的α-淀粉酶抑制蛋白基因打下了坚实的物质基础.“,”The genomic DNA from wheat variety 'Chinese Spring' was selected as the blocking DNA, and the genomic DNA from barley labelled with biotin-16-d UTP was used as in situ probe. Genetic composition of X99-13 from hybrids of CS × (CS +Betzes 2 H) was analyzed by genomic in situ hybridization. This material contained 42 chromosomes, including 1 barley chromosome, 2 wheat-barley translocation chromosomes and 39 wheat chromosomes, which were identified as wheat-barley substitution and translocation lines. The RFLP analysis was carried out by the short arm probe psr131 of the second part of the homologous group of wheat, the results of which showed that this substitution and translocation line involved the substitution and translocation of wheat chromosome 2 B and barley chromosome 2 H. This study laid a solid material foundation for further selection of the wheat-barley 2 H homozygous translocation line and utilization of the α-amylase inhibitor gene.