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贫血是慢性肾衰竭最常见和麻烦的并发症之一。虽然有很多因素可促使尿毒症贫血的发生,但大量证据表明,尿毒症贫血主要是由于肾病产生红细胞生成素(EPO)不足所致。人EPO系重糖基化的含166个氨基酸的多肽,分子量为34000。最近由于成功分离编码人EPO的基团,并在培养的哺乳动物细胞中克隆。这些细胞培养液内的EPO与天然的人EPO相似。此重组EPO已经纯化,在动物表明无毒性,并以典型的剂量依赖性方式刺激红细胞生成。人重组EPO对血液透析病人的效果 Eschbach等对25例红细胞压积(HCT)
Anemia is one of the most common and troublesome complications of chronic renal failure. Although there are many factors that contribute to the development of uremic anemia, there is ample evidence that anemia of uremia is primarily due to inadequate production of erythropoietin (EPO) by nephropathy. Human EPO is a heavily glycosylated 166 amino acid polypeptide with a molecular weight of 34,000. Recently, as a result of the successful isolation of the group coding for human EPO, it was cloned in cultured mammalian cells. EPO in these cell culture media is similar to native human EPO. This recombinant EPO has been purified, shows no toxicity in animals and stimulates erythropoiesis in a typical dose-dependent manner. Effects of recombinant human EPO on hemodialysis patients Eschbach et al.