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本文报道了显色反应,沉淀反应和电泳分析等配合应用,鉴别中药方剂中是否含有当归的方法。作者分析了64种生药和22个含有当归的处方,其方法和结果如下: 取生药1g,加10ml水后于沸水浴上加热10分钟,过滤,滤液作供试液。取供试液一份点于醋酸纤维素膜上,在0.5%甲苯胺蓝——3%醋酸液中浸5分钟后,用1%醋酸洗、显色。64种生药中呈阳性反应的有42种,拟阳性反应的有9种。除黄芩外,均为淡红紫色斑点。在呈阳性反应的51种生药供试液中,加入0.6ml 5%十六烷基三甲基铵溴化物,待沉淀生成后离心,沉淀物用1ml 0.5M氯化钠溶液溶解(弃去不溶物),再加入4倍量乙醇,待沉淀生成后离心,沉淀用同量80%
This article reports the application of color reaction, precipitation reaction and electrophoresis analysis to identify whether the traditional Chinese medicine contains angelica. The author analyzed 64 crude drugs and 22 prescriptions containing Angelica sinensis. The methods and results were as follows: Take 1 g of the drug, add 10 ml of water and heat it on a boiling water bath for 10 minutes, filter, and use the filtrate as the test solution. Take a test solution on a cellulose acetate film, immersed in 0.5% toluidine blue - 3% acetic acid solution for 5 minutes, washed with 1% acetic acid, color. Among 42 kinds of crude drugs, 42 were positive, and 9 were positive. Except for yellow peony, all are reddish purple spots. In the 51 test liquids for positive reactions, 0.6 ml of 5% cetyltrimethylammonium bromide was added. After the precipitate was formed and centrifuged, the precipitate was dissolved in 1 ml of 0.5 M sodium chloride solution (discarded and insoluble). (substance), and then add 4 times the amount of ethanol until the precipitate is formed and centrifuged, and the sediment is 80% of the same amount.