利用体外试验测定耐氧性双歧杆菌(Bifidobacterium animalis subsp.lactis Qq08)的耐酸、耐胆汁特性;同时测定其对胆固醇的降解能力和对结肠癌腺细胞Caco-2的黏附性能。结果表明,该菌在pH 2.0条件下培养4 h,仍有84.38%的存活率;用1.0%胆汁培养6 h,该菌的存活率在90%以上。该菌对胆固醇的降解力为53.8%,对结肠癌腺细胞Caco-2的黏附数为(8.57±3.74)个/细胞。该菌可作为益生菌制剂和功能性乳制品的潜在菌株。 Bifidobacterium animalis subsp. Lactis Qq08 was tested for its acid-fast and bile-resistant properties by in vitro test. The cholesterol degrading ability and the adhesion to colon cancer gland cells Caco-2 were also determined. The results showed that the strain was cultured at pH 2.0 for 4 h and still had a viability of 84.38%. After cultured with 1.0% bile for 6 h, the viability of the strain was over 90%. The bacteria degradation of cholesterol was 53.8%, the number of colon cancer cells Caco-2 adhesion (8.57 ± 3.74) cells / cell. This bacterium serves as a potential strain for probiotic preparations and functional dairy products.