论文部分内容阅读
目的探讨肾衰宁对糖尿病大鼠肾脏的保护作用及可能机制。方法雄性Vista大鼠24只,随机选取8只作为正常对照组(N组),其余16只注射链脲佐菌素(STZ)制成糖尿病模型,并随机分为糖尿病肾病组(DN组,n=8)和肾衰宁治疗组(S组,n=8),给药8周。检测血糖、肾功能、24h尿蛋白定量等指标,并进行肾脏病理学检查及免疫组化测定肾组织中转化生长因子β1(TGF-β1)、层黏连蛋白(LN)及Ⅳ型胶原蛋白(ColⅣ)的表达。结果与N组相比,DN组和S组血糖、血肌酐、尿素氮、24h尿蛋白均明显增加(P<0.01),肾组织中TGF-β1、LN及ColⅣ表达明显升高(P<0.01);与DN组相比,S组除血糖外(P>0.05),其余指标均明显降低(P<0.01)。结论肾衰宁在糖尿病的肾损伤过程中具有保护作用,可降低尿蛋白,改善肾功能,并通过下调肾脏TGF-β1的表达,抑制细胞外基质(ECM)成分的积聚,从而延缓肾脏纤维化的进程。
Objective To investigate the protective effect of Shenshuaining on the kidney of diabetic rats and its possible mechanism. Methods Twenty-four male Sprague-Dawley rats were randomly divided into normal control group (n = 8) and streptozotocin (STZ). Sixteen diabetic diabetic nephropathy models were randomly divided into diabetic nephropathy group (n = = 8) and Shenshuaining treatment group (S group, n = 8) for 8 weeks. The levels of TGF-β1, LN and type Ⅳ collagen in renal tissue were detected by pathological examination and immunohistochemistry. Col IV) expression. Results Compared with group N, the levels of blood glucose, serum creatinine, urea nitrogen and 24h urinary protein in DN group and S group were significantly increased (P <0.01), while the expressions of TGF-β1, LN and ColⅣ in DN group were significantly higher than those in N group ); Compared with DN group, except for blood glucose in S group (P> 0.05), the other indexes were significantly lower (P <0.01). Conclusion Shenshuaining can protect renal function of diabetic rats, reduce urinary protein and improve renal function. It can delay the accumulation of extracellular matrix (ECM) and decrease renal fibrosis by down-regulating the expression of TGF-β1 The process.