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目的探讨耳甲腔刺激对药物难治性癫痫大鼠痫性发作及学习记忆的影响,明确特定频率电刺激癫痫大鼠耳甲腔引起的行为学改变。方法 120只Wistar雄性大鼠氯化锂-匹鲁卡品制备惯常发作的颞叶癫痫大鼠,苯巴比妥诱导建立并筛选药物难治性癫痫大鼠50只,随机分为刺激组(20只)、假刺激组(20只)、非刺激组(10只)。按特定参数进行刺激,刺激参数设定为:电流强度1mA,波宽500μs,频率20Hz,刺激时间30s,间歇5分钟,10h/d(08:00~18:00),连续刺激4周。24小时视频脑电监测大鼠发作情况,刺激结束后,进行Morris水迷宫实验,观察大鼠学习记忆能力变化。三组大鼠断头取脑后切片,HE染色,镜下观察。结果成功建立并筛选药物难治性癫痫大鼠,耳甲腔刺激组大鼠癫痫发作次数明显下降,非刺激组和假刺激组大鼠癫痫情况无明显改变。三组间癫痫发作情况差异有统计学意义(P<0.01);非刺激组和假刺激组刺激前后癫痫发作率无统计学意义(P>0.05)。与假刺激和非刺激组相比,刺激组大鼠定向航行试验潜伏期明显缩短(P<0.01),定向航行试验中刺激组大鼠穿越目标象限频次及目标象限停留时间均显著长于其他两组(P<0.01)。HE染色发现假刺激组和非刺激组海马神经元明显变性、减少,刺激组则较轻。结论电刺激耳甲腔不仅能够显著抑制大鼠痫性发作,同时能改善因反复痫性发作而受损的学习记忆能力。
Objective To investigate the effects of the stimulation of the cavum on the seizure and learning and memory of epilepsy rats with drug-refractory epilepsy, and to clarify the behavioral changes induced by electrical stimulation of the specific frequency in the caput cavity of epileptic rats. Methods A total of 120 Wistar male rats were treated with lithium-pilocarpine to prepare the dominant temporal lobe epilepsy rats. Phenobarbital induced the establishment and screening of 50 refractory epilepsy rats, and were randomly divided into stimulation group (20 Only), sham stimulation group (20), non-stimulation group (10). The stimulation parameters were set according to the specific parameters: current intensity 1mA, wave width 500μs, frequency 20Hz, stimulation time 30s, intermittent 5 minutes, 10h / d (08:00 ~ 18:00), continuous stimulation for 4 weeks. 24 hours video EEG monitoring rat seizure situation, after the stimulation, Morris water maze test to observe the changes in learning and memory ability. Three groups of rats were decapitated brain sections, HE staining, microscopic observation. Results The successful establishment and screening of drug-refractory epilepsy rats, the number of epileptic seizures decreased significantly in the cavum-constipation-stimulated group, and there was no significant change in the epilepsy in the non-stimulated group and the sham-stimulated group. There were significant differences in seizures between the three groups (P <0.01). There was no significant difference in the seizure rate between the non-stimulated group and the sham-stimulated group (P> 0.05). Compared with the sham-stimulated and non-stimulated groups, the latency of the directional navigation test in the stimulation group was significantly shorter (P <0.01). In the directional navigation test, the rats in the stimulation group crossed the target quadrant frequency and the target quadrant residence time was significantly longer than the other two groups P <0.01). HE staining showed that the hippocampal neurons in the sham-stimulated group and non-stimulated group were significantly degenerated and decreased, while the stimulated group was lighter. Conclusion The electrical stimulation of the ear cavity can not only significantly inhibit the seizure in rats, but also improve the learning and memory abilities that are impaired by recurrent seizures.