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目的 :探讨藻蓝蛋白色素肽的理化特性及光动力疗法 (PDT)抗肿瘤效果。方法 :用柱层析法从螺旋藻藻蓝蛋白酶介产物中分离色素肽 ,并用 MTT法及口丫啶橙染色法检测色素肽对体外培养的小鼠肉瘤细胞 S180及荷瘤小鼠 PDT作用效果。结果 :分离出三种色素肽的分子量分别为 17.4KD、7.72 KD及 6 .6 KD,它们的吸收光谱峰值分别位于 6 18nm ,5 80 nm ,6 0 5 nm,荧光发射峰均在 6 85 nm处。 MTT法检测证明 ,在浓度为 10 0︼g/ m l,用波长为 5 80 nm、6 0 0 nm,照射剂量为 2 8.8J/ cm2染料激光 (氩离子为激发光源 )照射 ,对肿瘤细胞的杀伤率达 6 9.2 %～ 80 .2 %;在色素肽CCP1 、CCP3 介导的 PDT对小鼠移植瘤生长影响的实验发现 ,色素肽易被瘤细胞吸收 ,当激光照射剂量为 12 0 J/cm2 ,肿瘤直径在 0 .5～ 0 .7cm范围内 ,瘤体旁注射剂量为 5 0 ︼g的 CCP1 及 CCP3 光敏剂时 ,藻胆蛋白酶介产物的抑瘤率可达 46 %～ 81%,且被 PDT作用后的细胞表现了典型的凋亡细胞特征。结论 :藻蓝蛋白色素肽具良好的 PDT效果 ,有可能通过诱发凋亡途径导致细胞死亡。
Objective: To investigate the physicochemical properties of phycocyanin peptide and the anti-tumor effect of photodynamic therapy (PDT). Methods: The peptide was separated from spirulina phycocyanin by column chromatography. The effect of peptide on S180 and PDT in tumor-bearing mice was detected by MTT assay and acridine orange staining . RESULTS: The molecular weights of the three peptides were 17.4KD, 7.72 KD and 6.6KD, respectively. The peaks of their absorption spectra were located at 6 18nm, 580 nm and 655 nm, respectively. The fluorescence emission peaks were all at 6 85 nm Department. MTT assay showed that the concentration of 10 0 ︼ g / ml, with a wavelength of 5 80 nm, 600 nm, irradiation dose of 8.88 J / cm2 dye laser (argon ions as excitation light source) irradiation on tumor cells Killing rate of 62.92% ~ 80.2%; in the pigment peptide CCP1, CCP3-mediated PDT mice transplanted tumor growth experiments found that pigment peptide easily absorbed by tumor cells when the laser irradiation dose of 12 0 J / cm2, tumor diameter in the range of 0.5 ~ 0. 7cm, the tumor adjacent to the injection dose of 50 ︼ g of CCP1 and CCP3 photosensitizer, the phytoesterase-mediated inhibition rate of up to 46% to 81% The cells treated with PDT showed typical characteristics of apoptotic cells. CONCLUSION: Phycocyanin peptide has good PDT effect and may cause cell death by inducing apoptosis.