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Dopamine transporter (DAT) mediates the regulation of dopaminergic function. Two agents of TRODAT-1and FP-CIT were observed in evaluating DAT change of Parkinsons disease (PD). The relationship between them was also evaluated. The results suggested that 99mTc-TRODAT-1 SPECT and 131I-FP-CIT SPECT may serve as sensitiveand objective in vivo markers to reflect the severity of PD. The 99mTc-TRODAT-1 image is more accurate and clearercompared with 131I-FP-CIT.