论文部分内容阅读
为探讨间充质干细胞在成骨分化过程中的免疫原性改变及其意义,利用酶消化法获得胎盘间充质干细胞(PMSC),经鉴定后诱导成骨,用流式细胞术检测细胞表面协同刺激分子的表达并与T细胞共培养,3 H-TdR掺入法检测T细胞的增殖。结果表明,PMSC成功向成骨细胞分化,未分化的PMSC不表达CD28、CD80、CD83、CD86等正性共刺激分子,但经成骨诱导分化后其表达上调,并刺激了T细胞增殖,而未诱导分化的PMSC则未能促进。因此,未分化的PMSC具有低免疫原性,但是在经成骨诱导分化后其免疫原性上调,这对间充质干细胞今后在临床的应用有一定指导意义。
To explore the immunogenicity of mesenchymal stem cells during osteogenic differentiation and its significance, placental mesenchymal stem cells (PMSCs) were obtained by enzymatic digestion. After identification, osteogenic differentiation was induced and cell surface was detected by flow cytometry Costimulatory molecules and co-cultured with T cells, 3 H-TdR incorporation assay T cell proliferation. The results showed that PMSCs successfully differentiated into osteoblasts. Undifferentiated PMSCs did not express positive costimulatory molecules such as CD28, CD80, CD83, CD86 but upregulated after osteogenic differentiation and stimulated the proliferation of T cells The non-induced differentiation of PMSC failed to promote. Therefore, undifferentiated PMSC has low immunogenicity, but its immunogenicity is up-regulated after osteogenic differentiation, which may be of guiding significance for clinical application of mesenchymal stem cells in the future.