采用光度法研究了具抗凝血作用的过渡金属铁和铜的华法灵、水杨酸三元配合物与人血清白蛋白的相互作用,观察到铁、铜三元配合物使人血清白蛋白荧光产生猝灭现象,猝灭方式为静态猝灭,并计算了配合物与人血清白蛋白的结合常数和结合位点数.根据不同温度下的热力学函数,确定了配合物与人血清白蛋白的作用力类型.并且发现三元配合物的存在明显改变人血清白蛋白的构象.并讨论了配合物使人血清白蛋白构象发生变化的可能原因. The interaction of warfarin and salicylic acid ternary complexes with transition metal iron and copper with anticoagulant effect on human serum albumin was studied by spectrophotometry. It was observed that the iron and copper ternary complexes make human serum white The fluorescence quenching phenomenon of protein was quenched and quenched by static quenching, and the binding constants and binding sites of the complex with human serum albumin were calculated.According to the thermodynamic functions at different temperatures, the complexes with human serum albumin And found that the presence of the ternary complex significantly changed the conformation of human serum albumin and discussed the possible causes of the conformational change of human serum albumin by the complex.