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以2,3,4,6-乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯(GITC)为柱前衍生化试剂,建立了盐酸洛美沙星对映体柱前衍生化RP-HPLC拆分方法。采用了C18(4.6×250mm,5μm,Bnentnach)为色谱柱,在流动相为甲醇∶(3mmol.L-1四丁基溴化铵水溶液∶5mmol.L-1Na2HPO4的水溶液=1∶2)=25∶75,流速为1mL·min-1,检测波长284nm的色谱条件下,盐酸洛美沙星的非对映体在1.0—27.5μg.mL-1的浓度范围内有良好的线性关系,日内、日间精密度都<3%,且获得了基线分离。该种方法能用于盐酸洛美沙星的手性拆分,也为盐酸洛美沙星的光学异构体的测定提供了参考。
To establish a precolumn derivatization RP-HPLC method for the determination of lomefloxacin hydrochloride using 2,3,4,6-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) as pre-column derivatization reagent, HPLC resolution method. C18 (4.6 × 250mm, 5μm, Bnentnach) was used as the column. The mobile phase consisted of methanol: (3mmol.L-1 tetrabutylammonium bromide aqueous solution: 5mmol.L-1Na2HPO4 in water = 1: 2) = 25 : 75, the flow rate of 1mL · min-1, the detection wavelength of 284nm under the chromatographic conditions, lomefloxacin diastereomer in the concentration range of 1.0-27.5μg.mL-1 has a good linear relationship between days, Japan Inter-precision <3%, and obtained baseline separation. The method can be used for the chiral resolution of lomefloxacin hydrochloride and also provides a reference for the determination of optical isomers of lomefloxacin hydrochloride.