论文部分内容阅读
目的建立采用氯化钯为基体改进剂测定人体尿液中铬的石墨炉原子吸收方法。方法尿样用1%稀硝酸稀释后,氯化钯作为基体改进剂进行石墨炉原子吸收法测定尿中铬的含量。研究方法按照《生物材料中化学物质测定方法》的要求进行。结果筛选出氯化钯作为测定人体尿液中铬的基体改进剂。该方法检测铬时在0~40μg/L范围内呈线性关系,浓度与吸光度的回归方程为Y=0.017 6X+0.036 5;相关系数为0.999 7,最低检出限为0.353μg/L;不同浓度的相对标准偏差为0.28%~1.97%,加标回收率在97.9%~102.0%之间。样品在试管中分别于-20和4℃环境下保存,在1~14 d内均保存良好。结论采用氯化钯作为基体改进剂,用于石墨炉原子吸收光谱法测定尿液中的铬元素,该方法具有灵敏度高、稳定性好、操作简便、检出限低等特点,适用于尿中铬含量的测定。
Objective To establish a graphite furnace atomic absorption method for the determination of chromium in human urine with palladium chloride as matrix modifier. Methods The urine sample was diluted with 1% dilute nitric acid and the content of chromium in the urine was determined by graphite furnace atomic absorption spectrometry after using palladium chloride as matrix modifier. Research methods in accordance with “Determination of chemical substances in biological materials” requirements. Results As a matrix modifier for the determination of chromium in human urine, palladium chloride was screened out. The calibration curve was linear in the range of 0-40 μg / L. The regression equation of concentration and absorbance was Y = 0.017 6X + 0.036 5, the correlation coefficient was 0.999 7, the lowest detection limit was 0.353 μg / L, The relative standard deviations (RSDs) ranged from 0.28% to 1.97% with recoveries of 97.9% -102.0%. Samples were stored in tubes at -20 and 4 ° C, respectively, and stored well within 1 to 14 days. Conclusion Palladium chloride was used as a matrix modifier for the determination of chromium in urine by graphite furnace atomic absorption spectrometry. The method has the characteristics of high sensitivity, good stability, simple operation and low detection limit. It is suitable for urine Determination of chromium content.