论文部分内容阅读
目的:建立稳定的可卡因固定比率自身给药大鼠模型,并提高其成功率。方法:16只大鼠随机分为对照组和模型组,每组8只。2组大鼠全部行右侧颈静脉长期置管术,术后第6天起进行每天2小时的自身给药训练,训练程序为固定比率的FR1程序(即大鼠触碰鼻触一次可获得药物注射一次),模型组注射药物为浓度为5mg/ml的可卡因溶液(50μl/次),对照组为生理盐水(50μl/次),每次注射后20秒内为不应期,当大鼠连续3天触鼻频率最高值与最低值的差值小于均数标准差的1/4后,FR1模式下的大鼠自身给药训练成功。结果:通过8-11天训练,模型组8只SD大鼠全部形成稳定的自身给药行为,且与对照组相比,触鼻次数明显增加,P<0.01。结论:通过静脉自身给药训练,盐酸可卡因可使大鼠建立稳定的自身给药模型。通过改善手术质量、加强术后维护,可明显提高模型成功率。
OBJECTIVE: To establish a stable cocaine-administered rat model by itself and to increase its success rate. Methods: Sixteen rats were randomly divided into control group and model group, with 8 rats in each group. Long-term catheterization of the right jugular vein was performed in both groups, and self-administration was performed 2 hours a day after the sixth day. The training procedure was a fixed ratio FR1 program (ie, Drug injection once). The model group was injected with cocaine solution (50μl / time) with the concentration of 5mg / ml and the control group with normal saline (50μl / time). When the rats After 3 days, the difference between the maximum and minimum values of the nasal contact frequency was less than 1/4 of the standard deviation of the mean. The rats in the FR1 model were self-administered successfully. Results: After 8-11 days of training, all eight SD rats in the model group formed stable self-administration behavior. Compared with the control group, the number of nasal passages significantly increased (P <0.01). CONCLUSION: Cocaine hydrochloride can establish a stable self-administration model by intravenous self-administration. By improving the quality of surgery and strengthening postoperative maintenance, the model success rate can be significantly improved.