This work describes the development,optimisation and validation of an analytical method for the rapid determination of 17 priority pharmaceutical compounds and endocrine dis-rupting chemicals(EDCs).Rather than studying compounds from the same therapeutic class,the analyses aimed to determine target compounds with the highest risk potential(with particular regard to Scotland),providing a tool for further monitoring in different water matrices.Prioritisation was based on a systematic environmental risk assessment approach,using consumption data;wastewater treatment removal efficiency;environmental occur-rence;toxicological effects;and pre-existing regulatory indicators.This process highlighted 17 compounds across various therapeutic classes,which were then quantified,at environ-mentally relevant concentrations,by a single analytical methodology.Analytical determi-nation was achieved using a single-step solid phase extraction(SPE)procedure followed by high-performance liquid chromatography with tandem mass spectrometry(HPLC-MS/MS).The fully optimised method performed well for the majority of target compounds,with re-coveries ＞71％for 15 of 17 analytes.The limits of quantification for most target analytes(14 of 17)ranged from 0.07 ng/L to 1.88 ng/L in river waters.The utility of this method was then demonstrated using real water samples associated with a rural hospital/setting.Eight com-pounds were targeted and detected,with the highest levels found for the analgesic,parac-etamol(at up to 105,910 ng/L in the hospital discharge).This method offers a robust tool to monitor high priority pharmaceutical and EDC levels in various aqueous sample matrices.