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目的:观察地骨皮对实验性皮肤损伤与炎症反应的影响。方法:采用透明质酸酶抑制试验观察地骨皮不同提取物的体外抗炎效应;采用蒸汽烫伤制备小鼠深Ⅱ度皮肤烫伤模型,烫伤局部涂抹给药,检测创面等面积硫酸纸重、计算愈合天数、皮肤HE病理染色及羟脯氨酸含量,观察地骨皮对皮肤创面愈合作用。结果:地骨皮水提物、乙醇提取物对透明质酸酶的活性有抑制效果;地骨皮乙醇提取物能促进小鼠烫伤受损皮肤愈合,修复受损皮肤结构;在皮肤创伤修复第15天,地骨皮乙醇提取物、石油醚提取物和氯仿提取物能提高皮肤羟脯氨酸含量,促进胶原沉积和皮肤修复。结论:地骨皮具有明确的抑制皮肤炎症反应、促进小鼠烫伤皮肤创面愈合作用,但其修复皮肤损伤的确切机制及有效成分仍须深入研究。
Objective: To observe the effect of Digupi on experimental skin injury and inflammatory reaction. Methods: The hyaluronidase inhibition test was used to observe the anti-inflammatory effect of different extracts of Digupi in vitro. The scald model of deep second-degree scald skin was made by steam scald and scald, Healing days, HE staining and hydroxyproline content in skin were observed. The healing effect of Digupi on skin wounds was observed. Results: The extract of Dioscorea nipponica and ethanol extract had inhibitory effect on the activity of hyaluronidase. Ethanol extract of Digupi could promote the healing of scalded skin in mice and repair the damaged skin structure. In skin wound repair 15 days, Digupi ethanol extract, petroleum ether extract and chloroform extract can increase the hydroxyproline content of the skin, promote collagen deposition and skin repair. CONCLUSION: Digupi can obviously inhibit the inflammatory reaction of the skin and promote the wound healing of scalded skin in mice. However, the exact mechanism and active ingredients for repairing skin lesions still need further study.