【摘 要】
：
应用Dienes′染色剂的0.2％溶液于徒手切片或冰冻显微切片显出染病植株韧皮部中的类菌原体(MLO)。这种染色剂对MLO病害,包括一些螺旋体病原表现出特异性。而在其它病原侵染的植
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应用Dienes′染色剂的0.2％溶液于徒手切片或冰冻显微切片显出染病植株韧皮部中的类菌原体(MLO)。这种染色剂对MLO病害,包括一些螺旋体病原表现出特异性。而在其它病原侵染的植物组织中没有反应。手续是很快的,并具有诊断价值。
Mycoplasma (MLO) in the phloem of diseased plants was visualized on a freehand or frozen microsection using a 0.2% solution of Dienes’ stain. This stain shows specificity for MLO diseases, including some spirochetes. In other pathogen infected plant tissue no response. Procedures are fast and have diagnostic value.
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