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目的对黄芩药材及其炮制品黄芩片进行HPLC指纹图谱研究。方法选择HPLC-UV指纹图谱。色谱条件:Hypersil C18柱(200mm×5.0mm,5μm);流动相为甲醇-0.4%磷酸水溶液-乙腈线性梯度洗脱;流速:1.0mL·min-1;柱温28℃;检测波长277nm。结果10批不同产地正品黄芩进行规范炮制加工后得到的黄芩片指纹图谱无明显差异,14个不同产地黄芩药材的指纹图谱也具有极高的相似度。结论该方法重复性好,可为黄芩药材及其饮片的质量控制提供科学依据。
Objective To study the HPLC fingerprint of Radix Astragali and its processed products, Huangqi tablets. Methods HPLC-UV fingerprints were selected. Chromatographic conditions: Hypersil C18 column (200mm×5.0mm, 5μm); mobile phase is methanol-0.4% phosphoric acid aqueous solution-acetonitrile linear gradient elution; flow rate: 1.0mL·min-1; column temperature 28°C; detection wavelength 277nm. Results There were no significant differences in the fingerprints of the Huangqi tablets obtained after standard batch processing of 10 batches of authentic Astragalus membranaceus from different origins. The fingerprints of Radix Astragali from 14 different origins also had a very high similarity. Conclusion The method has good reproducibility and can provide scientific basis for quality control of Astragalus membranaceus and its decoction pieces.