用低温诱导培养的方法对夜蛾斯氏线虫Ａ５４的适低温特性进行了选育。结果表明，Ａ５４品系依序经过２５、２０、１５、１０℃４个温度梯度，每个温度连续培养３个侵染循环后，在１０℃下的侵染力有显著提高，对大蜡螟幼虫的致死速度加快，侵染周期缩短。与在２５℃下培养相比，侵入率提高了１０１９％，ＬＴ５０和侵染周期分别缩短了７４８ｈ和５ｄ。但返回在２５℃下培养２个侵染循环后，其适低温特性均有不同程度的丢失，侵入率比在１０℃培养下降低了２８３％，ＬＴ５０和侵染周期延长了３５ｈ和１４８ｄ。 Low temperature induction culture method was used to select the suitable low temperature characteristics of C. carinata A54. The results showed that the A54 strain was subjected to four temperature gradients at 25, 20, 15 and 10 ℃ in sequence. After three successive incubation cycles at each temperature, the infectivity at 10 ℃ was significantly increased, The lethal speed, infection cycle shortened. Compared with the culture at 25 ℃, the invasion rate increased by 1019%, LT50 and infection cycle were shortened by 748h and 5d respectively. However, after 2 cycles of infection at 25 ℃, the low temperature characteristics were all lost to different degrees, the invasion rate was reduced by 283% and the LT50 and infection cycle were prolonged by 35h and 14, respectively  8d.