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为深入研究罗汉果中功能成分的药理药效,进一步开发罗汉果中的有效成分,将罗汉果鲜果的水提物以AB 8吸附树脂分离、D 280离子交换树脂脱色后,利用半制备高效液相色谱法得到三萜皂甙类化合物罗汉果甜甙Ⅴ标准品,纯度达98 5%。色谱柱为AlltechEconosphereNH2柱;流动相为乙腈 水(体积比为68∶32)溶液,流速5mL/min;检测波长203nm;柱温40℃。此方法具有操作简便、重现性好、产品纯度高等优点。
In order to further study the pharmacological effects of the functional components in Mangosteen, further develop the active ingredients in Mangosteen, separate the aqueous extracts of fresh Mangosteen fruit with AB 8 adsorption resin, decolorize the D 280 ion exchange resin, use half-preparative high performance liquid chromatography Triterpenoid glycosides mogroside Ⅴ standard grade, purity 98 5%. The column was Alltech Econosphere NH2 column; the mobile phase was acetonitrile (volume ratio 68:32), the flow rate was 5 mL / min; the detection wavelength was 203 nm; the column temperature was 40 ℃. This method has the advantages of easy operation, good reproducibility and high product purity.