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目的研究胡椒酸己二胺(PAH)对实验性高脂血症大鼠的降血脂作用。方法雄性SD大鼠78只,适应性饲养1w后,测定基础血清胆固醇(TC)和甘油三酯(TG),随机抽取12只作为正常对照组,给予普通饲料,余作为造模组喂以高脂饲料。喂养4w后,再次检测血清TC和TG,比较造模前、后的血脂水平,以TC、TG显著升高为造模成功。将造模成功的大鼠按血清TC和TG水平分层,并随机分为高脂模型组、辛伐他汀阳性药物组(10mg/kg体质量)和PAH高剂量(10mg/kg体质量)、中剂量(5mg/kg体质量)、低剂量(2.5mg/kg体质量)组。每日灌胃给予各受试物,第4周末,麻醉动物,采集血清,测定TC、TG、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),同时制作石蜡切片,光镜观察各组大鼠肝脏组织形态变化。结果 PAH中、高剂量组血清TC水平较高脂模型组低,LDL-C水平较阳性药物组降低,差异均有统计学意义(P<0.05);PAH各剂量组血清TG、LDL-C水平均较高脂模型组低,差异具有统计学意义(P<0.05);低剂量组HDL-C水平较高,差异具有统计学意义(P<0.05)。PAH各剂量组血清TC、LDL-C水平存在剂量-效应关系,在高剂量时差异最为显著。中剂量PAH可明显改善肝细胞肿胀情况,对肝细胞有一定的保护作用。结论胡椒酸己二胺具有降血脂以及保护肝脏的作用。
Objective To study the hypolipidemic effects of captopril hexamethylenediamine (PAH) in experimental hyperlipidemic rats. Methods Seventy-eight male Sprague-Dawley rats were used for feeding for one week. The basal serum cholesterol (TC) and triglyceride (TG) were measured. Twelve mice were randomly selected as normal control group and fed with normal diet. Fat feed. After feeding for 4 weeks, the serum TC and TG were tested again. The level of blood lipid was compared before and after the model was established, and the TC and TG were significantly increased for modeling success. The successful rats were stratified by serum TC and TG levels and were randomly divided into high fat model group, simvastatin positive group (10mg / kg body weight) and PAH high dose (10mg / kg body weight) Medium dose (5 mg / kg body weight), low dose (2.5 mg / kg body weight) group. At the end of the fourth week, the animals were anesthetized and the serum was collected for the determination of TC, TG, HDL-C and LDL-C, and paraffin The morphological changes of liver tissue in each group were observed by light microscope. Results The levels of serum TC in middle and high-dose PAH groups were lower than those in the high-fat model group and LDL-C levels were lower than those in the positive group (P <0.05). The levels of TG and LDL-C (P <0.05). The levels of HDL-C in the low-dose group were higher, the difference was statistically significant (P <0.05). There was a dose-effect relationship between serum TC and LDL-C levels in PAH groups and the most significant difference at high doses. Medium dose PAH can significantly improve the swelling of hepatocytes, hepatocytes have a protective effect. Conclusions Pepper acid, hexamethylenediamine has the effect of lowering blood fat and protecting the liver.