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目的:建立何首乌二苯乙烯部位质量控制方法。方法:采用紫外分光光度法测定总二苯乙烯含量,采用高效液相色谱法测定2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量。结果:以2,3,5,4,-四羟基二苯乙烯-2-O-β-D-葡萄糖苷为对照品,测定总二苯乙烯含量时,在3.04-15.20μg/ml范围内线性关系良好,回归方程为Y=0.0588X+0.0026(r=0.9998),平均加样回收率为99.95%,RSD=2.11%(n=6);测定2,3,5,4,-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量时,在0.1032-0.6192μg范围内线性关系良好,回归方程为Y=3614089.84X+14475.21(r=0.9997),平均加样回收率为100.08%,RSD=2.83%(n=6)。结论:3批样品含量测定结果表明,所建立的方法简便、准确,可用于何首乌二苯乙烯部位质量控制。
Objective: To establish a quality control method for the stilbene site of Polygonum multiflorum. Methods: The total stilbene content was determined by UV spectrophotometry. The content of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside was determined by HPLC. RESULTS: When 2,3,5,4,-tetrahydroxystilbene-2-O-β-D-glucoside was used as a reference substance, the total stilbene content was linear in the range of 3.04-15.20 μg/ml. The relationship was good, the regression equation was Y=0.0588X+0.0026 (r=0.9998), the average recovery was 99.95%, RSD=2.11% (n=6), and 2,3,5,4-tetrahydroxyl was determined. When styrene-2-O-β-D-glucoside content was in the range of 0.1032-0.6192μg, the linear relationship was good, the regression equation was Y=3614089.84X+14475.21 (r=0.9997), and the average recovery was 100.08%. RSD = 2.83% (n = 6). Conclusion: The determination results of three batches of samples showed that the established method is simple and accurate, and can be used for the quality control of the stilbene site of Polygonum multiflorum.