In recent years,research in life sciences has been remarkably revolutionized owing to the establishment,development and application of genome editing technologies.Genome editing has not only accelerated fundamental research but has also shown promising applications in agricultural breeding and therapy.In particular,the clustered,regularly interspaced,short palindromic repeat (CRISPR) technology has become an indispensable tool in molecular biology owing to its high efficacy and simplicity.Genome editing tools have also been established in silkworm (Bombyx mori),a model organism of Lepidoptera insects with high economic importance.This has remarkably improved the level and scope of silkworm research and could reveal new mechanisms or targets in basic entomology and pest management studies.In this review,we summarize the progress and potential of genome editing in silkworm and its applications in functional genomic studies for generating novel genetic materials.