论文部分内容阅读
目的探讨从肝癌患者外周血中大量快速分离树突状细胞（ＤＣ）有效方法。方法自肝癌患者外周血中分离出单个核细胞（ＰＢＭＣ）；ＰＢＭＣ与Ｇｍ－ＣＳＦ及ＩＬ－４共培养；检测培养前后ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７－２表达水平及ＤＣ诱导Ｔ细胞增殖能力。结果ＧＭ－ＣＳＦ及ＩＬ－４联合刺激选择性使ＰＢＭＣ中ＤＣ大量增殖，并通过增强ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７表达［从（１２．８±１．１）、（１５．１±１．０）增至１９．１±１．７）、（２１．６±１．５），Ｐ＜０．０１］进一步增强ＤＣ免疫功能［由（６８２０±１４０）增至（１４０９０±１８０）ｍｉｎ－１，Ｐ＜０．０１］。结论联合应用ＧＭ－ＣＳＦ及ＩＬ－４能够从肝癌患者血中制备出大量高免疫活力ＤＣ。
Objective To explore a rapid method for rapid isolation of dendritic cells (DCs) from peripheral blood of patients with liver cancer. Methods Mononuclear cells (PBMCs) were isolated from peripheral blood of patients with hepatocellular carcinoma. PBMCs were co-cultured with Gm-CSF and IL-4. The expression of HLA-DR and B7-2 on DC surface and DC-induced T cell proliferation were measured before and after culture. Results The combined stimulation of GM-CSF and IL-4 stimulated the proliferation of DC in PBMCs, and enhanced the expression of HLA-DR and B7 on DC surface [from (12.8±1.1), (15.1±1.0) ) increased to 19.1±1.7) and (21.6±1.5), P<0.01] further enhanced DC immune function [from (6820±140) to (14090±180) min-1 P<0.01]. Conclusion The combination of GM-CSF and IL-4 can produce a large amount of high immune activity DC from the blood of patients with liver cancer.