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本文介绍一种特异、灵敏、简便测定人载脂蛋白CⅢ(apoCⅢ)的竞争性酶免疫测定法。单价特异抗体由免疫家兔获得。部分纯化的apoCs用于包被聚苯乙烯板。或根过氧化物酶-羊抗兔γ-球蛋白交联物按简化过碘酸钠法制备。apoCⅠ、CⅡ、AⅠ以及LDL(d=1.030～1.050g/ml)无交叉反应。本法最小检测量为100mg％。样品apoCⅢ含量在11.8～24.0mg％,板内及板间变异系数分别为6.8～7.5及8.2～10.7％,回收率为95.4％。35例正常人及24例高甘油三酯血症患者apoCⅢ含量分别为12.2±4.5及 27.5±5.7mg％(M±SD)。
This article describes a specific, sensitive, simple and convenient method for the determination of human apolipoprotein C (apoC Ⅲ) competitive enzyme immunoassay. Monovalent specific antibodies are obtained from immunized rabbits. Partially purified apoCs are used to coat polystyrene plates. Or peroxidase-goat anti-rabbit gamma-globulin cross-linker was prepared by a simplified sodium periodate method. apoCⅠ, CⅡ, AⅠ and LDL (d = 1.030 ~ 1.050g / ml). The minimum detection of this law is 100mg%. The apoCⅢ content of samples ranged from 11.8% to 24.0 mg%, the intraplate and interplate variation coefficients were 6.8-7.5 and 8.2-10.7%, respectively. The recovery rate was 95.4%. The apoCⅢ contents in 35 normal subjects and 24 hypertriglyceridemic patients were 12.2 ± 4.5 and 27.5 ± 5.7mg% (M ± SD), respectively.