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目的:建立测定利咽喷喉液中黄芩苷含量的高效液相色谱法。方法:采用Diamonsil C_(18)柱(150 mm×4.6 mm,5μm)分离,以甲醇-水-三乙胺(50:50:0.25,磷酸调节pH 2.5)进行洗脱,在277 nm处检测。结果:黄芩苷在10.06～80.48μg·ml~(-1)范围内质量浓度与峰面积呈良好的线性关系;黄芩苷的平均回收率为100.39%(RSD=0.74%,n=9)。结论:本法操作简便,准确度高,重复性好,可作为利咽喷喉液质量控制方法之一。
Objective: To establish a HPLC method for the determination of baicalin in Liyan Spray Liguid. METHODS: Diamonsil C_ (18) column (150 mm × 4.6 mm, 5 μm) was used for separation and eluted with methanol-water-triethylamine (50:50:25, pH 2.5). Results: The concentration of baicalin in the range of 10.06 ~ 80.48μg · ml ~ (-1) showed a good linear relationship with the peak area. The average recovery of baicalin was 100.39% (RSD = 0.74%, n = 9). Conclusion: This method is simple, accurate, reproducible and can be used as one of the quality control methods for Liyan throat liquid.