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应用高效液相色谱 电喷雾质谱联用法 ,直接对大豆胚芽的 70 %乙醇室温提取液进行检测 .根据各色谱峰的质谱特征 ,在室温提取液中鉴定出 9种异黄酮 ,7种A组皂苷 ,4种B组皂苷和2种E组皂苷 .结果证明对于复杂天然产物的定性定量分析 ,高效液相色谱 电喷雾质谱 (HPLC/ESI MS)联用分析法是一种方便快捷有效的方法 .
The high performance liquid chromatography-electrospray ionization mass spectrometry (ESI-MS / MS) method was used to detect the 70% ethanol extract of soybean germ directly.According to the mass spectral characteristics of each peak, nine isoflavones, seven kinds of group A saponins , Four kinds of Group B saponin and two kinds of Group E saponin.The results prove that HPLC / ESI MS combined analysis is a convenient and effective method for the qualitative and quantitative analysis of complex natural products.