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三维高效液相色谱（３Ｄ－ＨＰＬＣ）测定川芎煎剂及其灌胃大鼠后血清中均含有多种组分，其中一种用ＨＰＬＣ提纯后经紫外、红外光谱，质谱和核磁共振谱鉴定为川芎嗪（ＴＭＰ）。用ＨＰＬＣ法测定了大鼠灌胃川芎煎剂（Ｉ组，６只，３０ｇ／ｋｇ）和川芎丹参煎剂（Ⅱ组，６只，４０ｇ／ｋｇ，川芎：丹参＝３：１）后血清ＴＭＰ浓度，并研究了两组的药代动力学（药动学）。ＴＭＰ药动学参数中的一级吸收速度常数（Ｋａ）、与血药一时曲线下面积（ＡＵＣ）等值表明：Ⅱ组明显低于Ｉ组（Ｐ＜０．０５～０．０１），说明伍用丹参后引起ＴＭＰ吸收减慢和生物利用度降低。
Three-dimensional high-performance liquid chromatography (3D-HPLC) was used to determine the components of Chuanxiong decoction and its serum after intragastric administration. One of them was purified by HPLC and identified by ultraviolet and infrared spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. Tetramethylpyrazine (TMP). The serum TMP was determined by HPLC in rats of Chuanxiong Chuanxiong decoction (Group I, 6 rats, 30g/kg) and Chuanxiong Danshen decoction (Group II, 6 rats, 40g/kg, Chuanxiong: Salvia miltiorrhiza = 3:1). The concentrations and the pharmacokinetics (pharmacokinetics) of the two groups were studied. The first-order absorption rate constant (Ka) and the area under the curve of blood drug (AUC) in TMP pharmacokinetic parameters showed that group II was significantly lower than group I (P<0.05-0.01). After using Salvia Miltiorrhiza, TMP absorption slows down and bioavailability decreases.