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本实验选择对低温比较敏感的骨骼肌组织作为实验对象。将19只大鼠随机分成3组,其右后肢经冷冻处理使组织温度分别达+5℃、0℃及-5℃,于复温后不同时间测定骨骼肌中四种高能磷酸化合物AMP,ADP、ATP及CP的含量。结果表明,大鼠骨骼肌组织中高能磷酸化合物含量随冷冻时组织最低温度的下降而不同程度的减少,并且具有明显的时相过程。复温后即刻至4小时下降迅速,4～12小时三组值较为接近,其后随时间延长而明显分离。+5℃组恢复得早而且明显,0℃组恢复程度次于+5℃组,-5℃组含量下降最明显而且几乎不恢复。提示:低温使骨骼肌组织能量的产生和贮存过程受到影响。
In this experiment, skeletal muscle tissue that is sensitive to hypothermia was selected as the experimental subject. Twenty-nine rats were randomly divided into three groups, the right hind limbs were frozen and the tissues were heated to +5 ℃, 0 ℃ and -5 ℃, respectively. At the different time after rewarming, four kinds of high-energy phosphate compounds such as AMP, ADP , ATP and CP content. The results showed that the content of high-energy phosphoric acid in skeletal muscle tissue of rats decreased with the decrease of the minimum temperature of the frozen tissue, and the time-phase process was obvious. Immediately after rewarming to 4 hours decreased rapidly, 4 to 12 hours three values are closer, then significantly separated with time. The recovery of 5 ℃ group was earlier and obvious, the recovery degree of 0 ℃ group was less than that of + 5 ℃ group, the content of -5 ℃ group was the most obvious and hardly recovered. Tip: Low temperature causes skeletal muscle energy production and storage processes are affected.