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目的:观察当归芍药散(DSS)对垂体后叶素(Pit)所致小鼠心肌缺血的影响。方法:昆明种小鼠100只,分为空白对照组、模型组、DSS低剂量组(生药30 g.kg-1.d-1)、DSS高剂量组(生药60 g.kg-1.d-1)和阳性对照组(复方丹参滴丸0.174 g.kg-1.d-1),ig连续5 d,末次药后1 h,ip Pit(30 U.kg-1)进行造模。记录ip Pit前10 min和注射后5,15,25 min时点Ⅱ导联心电图;检测血清一氧化氮(NO)水平、内皮型一氧化氮合酶(eNOS)活性(免疫组化法)以及心肌组织匀浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:DSS高、低剂量组与模型组相比,心率均明显改善,具有显著差异(P<0.05或P<0.01);DSS高、低剂量血清NO水平分别为(79.35±25.18),(26.46±18.24)μmol.L-1,明显高于模型组(13.66±3.83)μmol.L-1(均P<0.01)。DSS高、低剂量eNOS活性为(0.259±0.037),(0.241±0.029),均强于模型组(0.176±0.036),P<0.01)。DSS高、低剂量心肌SOD活性分别为(269.55±20.65),(263.58±21.04)U.mg-1,明显高于模型组(222.75±23.44)U.mg-1(P<0.01);DSS高、低剂量心肌MDA含量分别为(3.58±1.01),(4.22±0.75)nmol.mg-1,明显低于模型组(5.25±1.42)nmol.mg-1(P<0.05或P<0.01)。结论:当归芍药散能有效防治由Pit所引起的心肌缺血及缺血损伤,具有保护心肌细胞的作用。
Objective: To observe the effect of Danggui Shaoyao San (DSS) on myocardial ischemia in mice induced by pituitary hormone (Pit). Methods: 100 Kunming mice were randomly divided into control group, model group, DSS low dose group (crude drug 30 g.kg-1.d-1), DSS high dose group (crude drug 60 g.kg-1.d -1) and positive control group (compound Danshen dripping pills 0.174 g.kg-1.d-1), ig for 5 consecutive days, and ip Pit (30 U.kg-1) for 1 h after the last dose. The electrocardiogram of Ⅱ lead at 10 min before ip Pit and at 5, 15, 25 min after injection were recorded. The level of serum nitric oxide (NO), the activity of eNOS (immunohistochemistry) Myocardial homogenate superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results: Compared with the model group, the heart rate of DSS high and low dose groups significantly improved (P <0.05 or P <0.01), and the levels of NO and NO in DSS high and low dose groups were (79.35 ± 25.18) and (26.46 ± 18.24) μmol.L-1, which was significantly higher than that of model group (13.66 ± 3.83) μmol.L-1 (all P <0.01). The activity of eNOS in high and low doses of DSS was (0.259 ± 0.037) and (0.241 ± 0.029), both of which were significantly higher than those in model group (0.176 ± 0.036, P <0.01). DSS high and low dose myocardial SOD activity were (269.55 ± 20.65), (263.58 ± 21.04) U.mg-1, significantly higher than the model group (222.75 ± 23.44) U.mg- 1 (P <0.01) (3.58 ± 1.01) and (4.22 ± 0.75) nmol.mg-1, respectively, which were significantly lower than those of model group (5.25 ± 1.42) nmol.mg-1 (P <0.05 or P <0.01). Conclusion: Danggui Shaoyao San can effectively prevent myocardial ischemia and ischemic injury caused by Pit and has the effect of protecting myocardial cells.