论文部分内容阅读
[目的]类风湿性关节炎(RA)、正常人(normal control)滑膜细胞体外原代培养,并检测三氧化二砷(arsenic trioxide,AS2O3)对滑膜细胞凋亡的作用过程中caspase3活性变化。[方法]双酶消化法体外培养人关节滑膜细胞,采用比色法对培养的滑膜细胞凋亡过程caspase3活性进行分析。[结果]比色法检测发现不同浓度的AS2O3作用48h后滑膜细胞裂解液中CASPASE-3的活性显著增强,并且在10～80μmol/L浓度范围内其作用呈一定剂量依赖性。[结论]caspase 3在AS2O3对滑膜细胞凋亡诱导过程中起到重要作用,并且其活性显著增强。
[Objective] To study the primary culture of synovial cells of rheumatoid arthritis (RA) and normal control in vitro and the change of caspase 3 activity during the process of synovial cell apoptosis induced by arsenic trioxide (AS2O3). [Method] Human synovial cells were cultured in vitro by double enzyme digestion method, and the activity of caspase3 in cultured synoviocytes apoptosis was analyzed by colorimetric method. [Result] The results of colorimetric assay showed that the activity of CASPASE-3 in synovial cell lysate was significantly increased after treated with different concentrations of AS2O3 for 48h, and its effect was dose-dependent in the range of 10 ~ 80μmol / L. [Conclusion] Caspase 3 plays an important role in the induction of synoviocyte apoptosis induced by AS2O3, and its activity is significantly enhanced.