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本文研究了大鼠急性和慢性CS_2接触,对脑组织高能磷酸盐化合物含量和线粒体形态结构的变化。材料和方法:选体重200～250 g雌性大鼠,分别连续接触CS_2蒸气,浓度为2.42mg/l12小时,或者浓度为0.8 mg/l,每日5小时、每周5天,共10个月。急性组在接触后立即处死,慢性组在末次接触后18小时处死。断头处死,使头直接落入液氮中。取出冻脑,在6%冰冷的过氯酸中制成匀浆,并调至中性。分别测定上清液中ATP(三磷酸腺苷)、ADP(二磷酸腺苷)、AMP(腺苷酸)和PC(磷酸
In this paper, we investigated the changes of high-energy phosphate compounds and mitochondrial morphology in acute and chronic CS 2 rats. MATERIALS AND METHODS: Female rats weighing 200-250 g were continuously exposed to CS_2 vapor at a concentration of 2.42 mg / l for 12 hours or at a concentration of 0.8 mg / l for 5 hours a day for 5 days a week for 10 months . The acute group was sacrificed immediately after the contact, and the chronic group was sacrificed 18 hours after the last contact. Decapitated, the head directly into the liquid nitrogen. Frozen brain was removed, homogenized in 6% chilled perchloric acid and adjusted to neutral. The concentrations of ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate) and PC (phosphate