Aim: To investigate the effect of air-conditioner exposure on semen quality. Methods: The data came from the healthy male volunteers, aged 22 to 30 years, who went to centers for maternity and children health for premarital physical examination in Shanghai, Henan, Zhejiang and Hebei from December 1998 to February 2000. The sampling size is 304. Results: Among the subjects, 90 (29.6 %) had air-conditioner at home and the rest did not. X2-test and multiple logistic regression analyses showed that, the difference between the exposure and control groups was statistically significant in semen volume, sperm density and proportion of sperm with normal morphology. The three indexes were lower in the exposure group. Conclusion: Air-conditioner exposure possibly influences the semen quality. Aim: To investigate the effect of air-conditioner exposure on semen quality. Methods: The data came from the healthy male volunteers, aged 22 to 30 years, who went to centers for maternity and children health for premarital physical examination in Shanghai, Henan, Zhejiang and Hebei from December 1998 to February 2000. The sampling size is 304. Results: Among the subjects, 90 (29.6%) had air-conditioner at home and the rest did not. X2-test and multiple logistic regression analyzes showed that, the difference between the exposure and control groups was quite significant in semen volume, sperm density and proportion of sperm with normal morphology. The three indexes were lower in the exposure group.