广泛存在于自然界中的多肽类物质,一般都具有明显的生物活性,随着现代科技的飞速发展,从天然产物中获取活性多肽的分离分析手段也不断得到提高,并出现一些新方法新思路。本文就近几年多肽类物质分离、分析的各种方法进展情况及其综合应用作一综述。1分离方法多肽提取分离常用的 Polypeptides widely existing in nature generally have obvious biological activities. With the rapid development of modern science and technology, the separation analysis methods for obtaining active peptides from natural products have also been continuously improved, and some new methods and new ideas have emerged. This article reviews the progress and comprehensive applications of various methods for the separation and analysis of peptides in recent years. 1 separation method polypeptide extraction and separation commonly used