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本研究考察了人参皂苷C-K(C-K)在体内外银屑病模型中的作用.在体外,检测了C-K在人永生化表皮细胞(HaCaT细胞)中的作用,以及在鸡胚绒毛尿囊膜(CAM)中对血管生成的作用.在体内采用4种银屑病模型,包括:己烯雌酚处理的小鼠阴道上皮细胞模型、鼠尾颗粒细胞层形成模型、普萘洛尔诱导的豚鼠耳皮肤以及咪喹莫特诱导的BALB/c小鼠背部皮肤中的银屑病样特征模型.结果 显示,C-K在体外可降低HaCaT细胞活力,抑制CAM血管生成.在体内,C-K改善了小鼠和豚鼠银屑病模型中的银屑病样组织学特征.C-K还可显著抑制小鼠阴道上皮细胞的有丝分裂,促进小鼠尾部鳞状上皮颗粒层的形成,降低普萘洛尔处理的豚鼠耳背侧角质层厚度;抑制了咪喹莫特诱导的BALB/c小鼠背部皮肤银屑病中皮肤增厚、水肿和炎性细胞浸润以及炎性因子的表达.据此可得出结论,C-K可以有效抑制银屑病动物模型,在临床研究中其对银屑病的治疗作用将得到进一步的探索应用.“,”The effects of ginsenoside compound K (C-K) were investigated within animal psoriasis models in vitro and in vivo.The effect of C-K was examined in HaCaT cells,while its effect on angiogenesis was tested in chick embryo chorioallantoic membrane (CAM) in vitro.The effectiveness of C-K in treating psoriasis was tested in four psoriasis models,including diethylstilbestrol-treated mouse vaginal epithelial cells,mouse tail granular cell layer formation,propranolol-induced psoriasis-like features in guinea pig ear skin and imiquimod-induced BALB/c mouse dorsal skin psoriasis in vivo.The results showed that C-K reduced HaCaT cell viability as well as suppressed CAM angiogenesis.Oral administration of C-K ameliorated psoriasis-like histological characteristics in both mouse and guinea pig psoriasis models.Moreover,C-K also significantly inhibited mouse vaginal epithelium mitosis,promoted mouse tail squamous epidermal granular layer formation,reduced the thickness of the horny layer in propranolol treated auricular dorsal surface of guinea pig,and inhibited the skin thickening,edema and inflammatory cell infiltration,and the expression of inflammatory factors in miquimod-induced BALB/c mouse dorsal skin psoriasis.It was concluded that C-K could effectively inhibit psoriasis in animal models.This study aims to investigate the effects of systematically applied C-K on psoriasis in animal models.Future clinical studies should be conducted to explore the therapeutic effects of C-K in humans.