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设置对照(CT)、增温5℃(W)、隔离50%降雨(P)和增温5℃+隔离50%降雨(WP)4种处理,以相关功能基因作为标志物,研究增温和隔离降雨影响亚热带森林生态系统土壤N_2O通量变化的途径.结果表明:隔离降雨显著降低了土壤铵态氮浓度;增温显著降低了土壤N_2O通量和土壤反硝化势.增温处理(W)和降雨处理(P)的土壤微生物生物量氮(MBN)均显著低于对照(CT),AOA amo A基因丰度与MBN和铵态氮含量之间呈显著负相关,但与土壤硝化势和土壤N_2O通量没有显著相关性.路径分析显示,反硝化势直接显著影响土壤N_2O通量,而微生物生物量磷(MBP)和增温则通过直接影响反硝化势来间接影响土壤N_2O通量.温度可能是影响亚热带森林土壤N_2O通量的主要驱动因素,全球变暖可能会减少亚热带森林土壤的N_2O排放.
Four treatments including CT, 5 ℃ (W), 50% rainfall isolation (P) and 5 ℃ warming (50% rainfall isolation) were used to study the effect of warming The results showed that: isolation rainfall significantly reduced soil ammonium nitrogen concentration; warming significantly reduced the soil N 2 O flux and soil denitrification potential. Temperature increase treatment (W) And soil microbial biomass nitrogen (MBN) of rainfall treatment (P) were significantly lower than that of control (CT). There was a significant negative correlation between AOA amo A gene abundance and MBN and ammonium nitrogen content, There was no significant correlation between soil N 2 O flux and pathological analysis. The results showed that the denitrification potential had a direct and significant effect on soil N 2 O flux, while MBP and warming indirectly affected soil N 2 O flux by directly affecting the denitrification potential. Temperature may be the main driver of N 2 O fluxes in subtropical forest soils, and global warming may reduce N 2 O emissions from subtropical forest soils.