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酶法拆分(±)-N-(2,6-二甲苯基)-丙氨酸甲酯,其反应体系为:在含1 mmol(±)-N-(2,6-二甲苯基)-丙氨酸甲酯的100 mL 0.2 mol.L-1磷酸缓冲液(PBS)中,添加2 g聚乙二醇(PEG-6000),并用皱落假丝酵母脂肪酶(Candida rugosalipases,CRL)拆分,反应后分离得到R-(+)-N-(2,6-二甲苯基)-丙氨酸甲酯。进一步采用高效液相色谱检测酶促反应的转化率,采用250 mm×4.6 mm,5μHypersil(ODS色谱柱,以甲醇与水混合液(体积比为80∶20)为流动相,230 nm为检测波长,外标法峰面积定量。(±)-N-(2,6-二甲苯基)-丙氨酸和其甲酯的回收率在91.5%~96.1%之间。
(±) -N- (2,6-xylyl) -alanine methyl ester by enzymatic resolution, the reaction system was as follows: -alanine methyl ester in 100 mL of 0.2 mol·L-1 phosphate buffer (PBS), 2 g polyethylene glycol (PEG-6000) was added and incubated with Candida rugosalipases (CRL) After resolution, R - (+) - N- (2,6-xylyl) -alanine methyl ester was isolated. The conversion of the enzymatic reaction was further measured by high performance liquid chromatography. The mobile phase was 250 mm × 4.6 mm, 5μHypersil (ODS column, methanol / water mixture (80:20) , And the peak area of external standard method was quantified.The recoveries of (±) -N- (2,6-dimethylphenyl) -alanine and its methyl ester ranged from 91.5% to 96.1%.