目的：从蟹壳中提取壳聚糖，将胰蛋白酶固定在从蟹壳中提取的壳聚糖上。方法：以自制壳聚糖为载体，戊二醛为双官能团交联剂，将胰蛋白酶固定化。结果：０．２ｇ壳聚糖（干）与４％戊二醛交联后再与５．０ｍｇ胰蛋白酶固定结合，效果较好。固定化酶的表现米氏常数Ｋ′ｍ＝１．５ｍｍｏｌ／Ｌ，而天然酶Ｋｍ＝７．６ｍｍｏｌ／Ｌ；固定化酶最适温度为８０℃，比天然酶提高了２０℃；固定化酶最适ｐＨ为７．０．而天然酶为８．０。该载体来源广，易提取，固定化酶的贮存稳定性很好，２个多月重复使用，酶活力未见明显下降 OBJECTIVE: To extract chitosan from crab shells and fix trypsin onto chitosan extracted from crab shells. Methods: Self-made chitosan as a carrier, glutaraldehyde as a bifunctional cross-linking agent, the trypsin immobilized. Results: 0.2g chitosan (dry) cross-linked with 4% glutaraldehyde and then fixed with 5.0mg trypsin, the better. The immobilized enzyme showed the Michaelis constant K’m = 1.5mmol / L, and the natural enzyme Km = 7.6mmol / L. The optimum temperature for the immobilized enzyme was 80 ℃, which was 20 ℃ higher than that of the natural enzyme. The immobilized enzyme The optimum pH is 7.0. The natural enzyme is 8.0. The vector source wide, easy to extract, the storage stability of the immobilized enzyme is very good, more than 2 months of repeated use, no significant decrease in enzyme activity