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目的:建立保济丸中葛根素含量的测定方法,评价几种灭菌方法的优劣。方法:以甲醇-水(30∶70)为流动相,流速1.0 mL.min-1,检测波长250 nm,柱温30℃,用RP-HPLC测定经各种灭菌方法处理的保济丸中葛根素的含量与未经灭菌处理的保济丸中葛根素的含量。结果:葛根素在4.212～84.24 mg.L-1呈良好的线性关系(r=0.999 9),平均回收率98.18%,RSD 0.44%。湿热蒸汽灭菌方法在4种灭菌方法中属最优法。结论:定量测定方法简便、快速、灵敏、结果准确、重复性好,可作为保济丸的质量控制标准。
Objective: To establish a method for the determination of puerarin in Baoji Pills and evaluate the advantages and disadvantages of several sterilization methods. Methods: The mobile phase was methanol-water (30:70) with the flow rate of 1.0 mL · min-1. The detection wavelength was 250 nm and the column temperature was 30 ℃. Puerarin contents and Puerarin content in Baoji Pills without sterilization. Results: Puerarin had a good linearity (r = 0.999 9) between 4.212-84.24 mg.L-1 with an average recovery of 98.18% and a RSD of 0.44%. Hot and humid steam sterilization methods in the four sterilization methods are the best method. Conclusion: The quantitative determination method is simple, rapid, sensitive, accurate and reproducible. It can be used as the quality control standard of Baoji Pills.