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[目的]分析血清胃蛋白酶原(PG)I、PGII、胰蛋白酶原-2(TAT2)、CEA、CA50、CA242指标在胃癌诊断中的应用价值。[方法]采用时间分辨荧光免疫分析法,检测116名胃癌患者和60名正常人血清PGI、PGII、CEA、CA50、CA242、TAT2含量,并计算PGI/PGII比值。[结果]血清PGI、PGI/PGII、CEA、CA50、CA242、TAT2与对照组比较结果在统计学上差异均有统计学意义,敏感度分别是34.48%、38.79%、12.93%、13.79%、26.72%和27.59%。而多项并联检测可有效提高敏感度,且特异度保持较高水平(均大于80%)。其中PGI、PGI/PGII和TAT23项联合敏感度为70.96%,再增加肿瘤标志物CEA、CA50与CA242全检,敏感度提高至84.02%,同时获得高的阳性似然比和低的阴性似然比。[结论]利用高通量TRFIA联合检测血清PGI、PGI/PGII、TAT2、CEA、CA50、CA242水平,能显著提高胃癌检出水平。
[Objective] To analyze the value of serum pepsinogen (PG) I, PGII, trypsinogen-2 (TAT2), CEA, CA50 and CA242 in the diagnosis of gastric cancer. [Methods] The serum levels of PGI, PGII, CEA, CA50, CA242 and TAT2 in 116 gastric cancer patients and 60 normal persons were detected by time-resolved fluorescence immunoassay and the PGI / PGII ratio was calculated. [Results] The results of serum PGI, PGI / PGII, CEA, CA50, CA242 and TAT2 were statistically significant difference compared with the control group, the sensitivity were 34.48%, 38.79%, 12.93%, 13.79%, 26.72 % And 27.59%. A number of parallel detection can effectively improve the sensitivity and specificity to maintain a high level (both greater than 80%). The combined sensitivity of PGI, PGI / PGII and TAT23 was 70.96%, and the tumor markers CEA, CA50 and CA242 were increased. The sensitivity was increased to 84.02%, and high positive likelihood ratio and low negative likelihood ratio. [Conclusion] Combined detection of serum PGI, PGI / PGII, TAT2, CEA, CA50 and CA242 by high-throughput TRFIA can significantly improve the detection of gastric cancer.