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生物破乳剂是一种用于油水分离的新型破乳剂.采用废弃柴油培养生物破乳剂产生菌Alcaligenes sp.S-XJ-1,培养7 d,菌株干重最高可达2.0 g/L,10 g/L的菌株细胞悬液能够将水表面张力从72.0 mN/m降低到29.7 mN/m.生物破乳剂产量为0.3 g/L,其CMC为150 mg/L,表面活性优于化学表面活性剂SDS,且对W/O模型乳状液的破乳效果在70%以上.废弃柴油GC-MS测试结果表明,S-XJ-1菌株能够利用废弃柴油中的C14～C20正构烷烃,且C20正构烷烃几乎被完全利用,其利用率高达99%.S-XJ-1菌株对不同碳链长度正构烷烃复配碳源的利用率及破乳性能随着碳链长度的延长而逐渐增加.与其他正构烷烃复配碳源相比,S-XJ-1菌株对C20正构烷烃利用率最高,合成破乳剂的性能最好,且与废弃柴油研究结果最为接近.TLC和FTIR分析表明S-XJ-1菌株利用废弃柴油合成的生物破乳剂为脂肽类物质.
Bio-demulsifier is a new type of demulsifier for oil-water separation.It was found that strain Alcaligenes sp.S-XJ-1 was cultured in waste diesel oil with bio-demulsifier, and its dry weight was up to 2.0 g / L and 10 g / L strain of cell suspension can reduce the surface tension of water from 72.0 mN / m to 29.7 mN / m.The bio-demulsifier yield is 0.3 g / L, the CMC is 150 mg / L, the surface activity is better than the chemical surfactant SDS, and the demulsification effect of W / O emulsion was over 70% .The results of GC-MS of waste diesel showed that S-XJ-1 strain could utilize C14 ~ C20 n-alkanes from waste diesel and C20 Almost completely, the utilization rate of paraffin was as high as 99% .S-XJ-1 strain’s utilization rate and demulsification performance of n-alkane with different carbon chain lengths increased gradually with the increase of carbon chain length. Compared with other n-alkane complexed carbon sources, S-XJ-1 strain had the highest utilization rate of C20 n-alkanes, and the best performance of the synthetic demulsifier was the closest to that of waste diesel oil.The analysis of TLC and FTIR showed that S -XJ-1 strain The bio-demulsifier synthesized from waste diesel oil is a lipopeptide.