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以鹿角菜胶（ＣＡＲ）注射到大鼠后爪皮下作为外周炎性刺激模型，用原位杂交结合免疫组化染色观察了ＰＰＴＡｍＲＮＡ在脊髓背角神经元中的表达变化及其与Ｆｏｓ蛋白阳性神经元的关系。结果显示：ＰＰＴＡｍＲＮＡ阳性神经元主要位于脊髓背角Ⅰ、Ⅱ层和Ⅴ、Ⅵ层。ＣＡＲ注射后ＰＰＴＡｍＲＮＡ阳性神经元的数量与对照侧相比明显增多。ＣＡＲ刺激也诱导了Ｆｏｓ蛋白在脊髓背角神经元中的表达。Ｆｏｓ阳性神经元主要位于刺激侧脊髓背角的Ⅰ、Ⅱ层和Ⅴ、Ⅵ层，在对照侧仅有少量分布。在脊髓背角各层均可见到ＰＰＴＡｍＲＮＡ和Ｆｏｓ阳性双标神经元。Ⅰ、Ⅱ层和Ⅴ、Ⅵ层中分别有７８％和８２％的ＰＰ－ＴＡｍＲＮＡ阳性神经元同时呈Ｆｏｓ阳性，而这两个区域中仅有２７％和３０％的Ｆｏｓ阳性神经元为双标神经元。
The carrageenin (CAR) was injected subcutaneously into the rat hind paw as a model of peripheral inflammatory stimuli. The expression of PPTA mRNA in spinal dorsal horn neurons was observed by in situ hybridization and immunohistochemistry. The relationship between yuan. The results showed that: PPTAmRNA positive neurons are mainly located in spinal dorsal horn Ⅰ, Ⅱ and Ⅴ, Ⅵ layer. The number of PPTA mRNA positive neurons after CAR injection increased significantly compared with the control side. CAR stimulation also induced the expression of Fos protein in spinal dorsal horn neurons. Fos positive neurons mainly located in the dorsal horn on the lateral spinal cord Ⅰ, Ⅱ and Ⅴ, Ⅵ layer, only a small amount of distribution on the control side. PPTA mRNA and Fos-positive double-labeled neurons were found in all layers of spinal dorsal horn. 78% and 82% of PP-TA mRNA positive neurons were found to be Fos-positive at the same time in I, II and V, and Ⅵ, while only 27% and 30% of Fos-positive neurons in these two regions were double-labeled Neurons.