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一、前言用人的淋巴细胞作某些致突变性指标的测定作为对人口健康危害的监测是很有意义的,目前已较为广泛的应用。但一般所使用的常规微量培养方法,每一培养也至少需0.3ml全血。仍然需要静脉穿刺取血,这在实际工作中仍会遇到阻力和带来操作过程中的麻烦和不便。因此很需要建立一种微量,简便,可靠而又经济的方法,参见“A True Microculture Technique For Hman Lymphocytes. Paul I Countryman”。Pane Countryman等介绍的微量淋巴细胞培养方法只要用10ml血,用微
I. INTRODUCTION The determination of certain mutagenic indicators of human lymphocytes is of great significance as a monitoring of the health risks of the population and has been widely used at present. However, the conventional micro-culture method generally used requires at least 0.3 ml of whole blood per culture. Still need to venipuncture blood, which in practice will still encounter resistance and bring the trouble and inconvenience in the operation. Therefore, it is necessary to establish a trace, simple, reliable and economical method, see “A True Microculture Technique For Hman Lymphocytes. Paul I Countryman”. Pane Countryman and other introduction of trace lymphocyte culture as long as 10ml blood, micro