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目的:建立测定复方庆大霉素合剂中盐酸普鲁卡因、盐酸麻黄碱、对羟基苯甲酸乙酯和硫酸庆大霉素的高效液相色谱法.方法:前3 种药物用反相色谱－ 波长和灵敏度切换法同时测定,硫酸庆大霉素用柱前衍生化色谱法测定.结果:质控结果显示药物浓度与峰高响应值线性关系良好,相关系数分别0－9995,0－9978,0－9966 和0－9992;4 种药物的加样回收率分别平均为95－5±5－6％ ,96－2±4－5％ ,96－0±4－7％ ,92－5±6－2％ ;日内RSD 均＜ 6－0 ％ ( N ＝5),连续测定5D 的日间RSD ＜ 7－2 ％( N ＝5),精密度和重现性良好.结论:该法适于作为药检室检测该合剂的方法“,”AIM To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of procaine hydrochloride,ephedrine hydrochloride,ethylparaben and gentamycin.METHODS The first three drugs were determined together within 15 minutes by a reverse phase HPLC with wavelength shearing and gentamycin by HPLC with derivatization before column.RESULTS There were good linear relationship between the 4 drugs concentration in mixture and chromatogram peak height with the interrelated coefficient 0 9995,0 9978,0 9966and 0 9992 The average add-sample recovery for 4 drugs were 95 5±5 6%,96 2±4 5%,96 0±4 7%,92 5±6 2%,respectively.Good precision and reproducibility were found with intra- and inter- day precisions being better than 6 0%and 7 2%.CONCLUSION The results showed that the methood was suitable to determined the 4 drugs in mixture.