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为了明确重要植物病原真菌黑白轮枝菌基因组中的微卫星(SSR)分布情况,利用软件SciRoKo从已测序的VaMs102菌株基因组(32.8 Mb)中,成功搜索得到4224个SSR。结果显示,三碱基重复微卫星的数量最多,为1481个,占SSR总数的35.1%;其次是二碱基、六碱基、四碱基和五碱基;单碱基重复的微卫星数量最少,为308个,仅占总数的7.3%。不同重复单元的微卫星在黑白轮枝菌基因组中的分布是非随机的,具有明显的偏好性。单碱基、二碱基、四碱基和五碱基偏爱出现于非编码区,尤其是基因间区。三碱基和六碱基重复微卫星较为集中分布在外显子区,并且重复次数变化丰富,推断黑白轮枝菌编码区的SSR可能会通过调节自身的重复次数来适应新环境。
In order to determine the microsatellite (SSR) distribution in the genome of the important phytopathogenic fungi, Verticillium dahliae, 4224 SSRs were successfully searched from the sequenced genome of VaMs102 strain (32.8 Mb) using the software SciRoKo. The results showed that the number of three-base repeat microsatellites was the highest, accounting for 1481, accounting for 35.1% of the total number of SSRs; followed by two bases, six bases, four bases and five bases; the number of single-base repeat microsatellites The minimum is 308, accounting for only 7.3% of the total. The distribution of microsatellites with different repeat units in the genome of Verticillium albus is non-random and has obvious preference. Monobasic, diacyl, tetrasitral and pentabasic preferences appear in non-coding regions, especially in intergenic regions. Three-base and six-base repeat microsatellites are more concentrated in the exon region, and the number of changes in the abundance of repetition, extrapolation of SSR may be able to adapt to the new environment by adjusting its own repetition number.