论文部分内容阅读
目的研究2-甲-4-氯原药(MCPA)的急性毒性和亚慢性毒性。方法急性毒性实验对大鼠经口灌胃进行单次染毒,观察动物中毒症状,计算半数致死剂量(LD50);亚慢性毒性实验分别以113.50、37.83、18.92 mg/kg不同剂量连续经口灌胃染毒13周,试验期间观察动物一般情况,记录每周体质量。试验结束时测血常规、血生化、尿常规、脏器系数及病理变化。结果MCPA大鼠急性经口LD50雌雄性动物均为681.00 mg/kg。亚慢性染毒后,113.50 mg/kg组动物出现明显中毒症状,体质量增长缓慢;血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活力升高,肝组织出现明显的病理改变;肾脏系数增大,动物尿中蛋白和红细胞阳性;37.83 mg/kg组动物出现明显中毒症状,部分动物尿蛋白和红细胞阳性;18.92 mg/kg组各项检测指标均未见异常。结论2-甲-4-氯原药属于低毒性物质,本实验条件下,大鼠90 d亚慢性经口最大无作用剂量为18.92 mg/kg。
Aim To study the acute toxicity and subchronic toxicity of 2-methyl-4-chlorophenol (MCPA). Methods Acute toxicity test was conducted in rats by oral gavage for a single dose. The symptoms of animal poisoning were observed and the LD50 was calculated. The sub-chronic toxicity test was conducted at different doses of 113.50, 37.83 and 18.92 mg / kg, respectively. Stomach poisoned for 13 weeks, during the observation of animals in general, record the weekly body mass. Test blood test at the end of routine, blood biochemistry, urine, organ coefficient and pathological changes. Results Acute oral LD50 in both male and female MCPA rats was 681.00 mg / kg. Sub-chronic exposure, 113.50 mg / kg group of animals showed significant symptoms of poisoning, body weight growth slow; serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity increased, the liver showed obvious pathology The kidney index increased, and urine protein and erythrocyte were positive in animals. The symptoms of poisoning were obvious in 37.83 mg / kg group and positive in urinary protein and erythrocyte in some animals. No abnormality was detected in 18.92 mg / kg group. Conclusions 2-Methoxy-4-chlorobenzaldehyde is a low toxicity substance. Under this experimental condition, the maximal non-acting dose of subchronic oral administration in rats for 90 days was 18.92 mg / kg.