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目的:研究昆布多糖(Laminarin)对结肠癌细胞生长、黏附和侵袭转移的抑制作用,为新药筛选提供理论依据。方法:采用同质、异质黏附,细胞分离及侵袭小室等试验技术检测不同含量昆布多糖对4种大肠癌细胞的细胞生物学特性的影响。结果:与对照组相比,在低含量药物作用时,基质黏附能力和同质黏附性,以及细胞分离能力均无明显变化。高含量药物作用后,sw480,sw620,HT29,LoVo基质黏附率分别为(0.292±0.21)%,(0.434±0.23)%,(0.428±0.43)%,(0.313±0.28)%,sw480,sw620,HT29,Lo-Vo同质黏附率分别为(10.6±2.3)%,(18.2±2.1)%,(25.5±3.0)%,(11.1±2.8)%,基质和同质黏附性均下降(P<0.05～0.01,t=4.217,7.105,10.571,2.196,8.245,3.452,10.563,5.231);sw480,sw620,HT29,LoVo细胞分离率增强,分别为(47.51±7.2)%,(45.26±8.3)%,(31.78±5.3)%,(45.226±7.5)%,(P<0.05～0.01,t=4.251,19.142,10.043,3.497);sw480,sw620,HT29,LoVo细胞穿过基底膜能力减弱,分别为(7.61±2.6),(20.4±3.1),(5.8±1.3),(15.1±3.6)个(P<0.01,t=11.541,12.534,16.143,23.427)。结论:昆布多糖可使细胞的恶性表型发生变化,使其侵袭转移能力受到抑制,并呈现剂量依赖性。
Objective: To study the inhibitory effect of Laminarin on the growth, adhesion and invasion and metastasis of colon cancer cells and to provide a theoretical basis for the screening of new drugs. Methods: Homogenization, heterogeneous adhesion, cell separation and invasive chamber techniques were used to test the effects of different concentrations of laminarin on the cell biological characteristics of four kinds of colorectal cancer cells. RESULTS: Compared with the control group, no significant changes were observed in the matrix adhesion and homogeneity, as well as in the cell separation ability at low levels of the drug. The adhesion rates of sw480, sw620, HT29 and LoVo were (0.292 ± 0.21)%, (0.434 ± 0.23)%, (0.428 ± 0.43)%, (0.313 ± 0.28)%, sw480, sw620, HT29 and Lo-Vo were (10.6 ± 2.3)%, (18.2 ± 2.1)%, (25.5 ± 3.0)% and (11.1 ± 2.8)%, respectively. (47.51 ± 7.2)% and (45.26 ± 8.3)%, respectively, which were significantly higher than that of the control group (P <0.05-0.01, t = 4.217,7.105,10.571,2.196,8.245,3.452,10.563,5.231) , (31.78 ± 5.3)%, (45.226 ± 7.5)%, respectively (P <0.05-0.01, t = 4.251,19.142,10.043,3.497). The ability of sw480, sw620, HT29 and LoVo cells to pass through the basement membrane was weakened (7.61 ± 2.6), (20.4 ± 3.1), (5.8 ± 1.3) and (15.1 ± 3.6), respectively (P <0.01, t = 11.541,12.534,16.143,23.427). CONCLUSION: Laminarin can change the malignant phenotype of the cells, inhibiting the invasion and metastasis and exhibiting a dose-dependent manner.