为了探讨肺炎支原体（Ｍｐｎ）和生殖支原体（Ｍｇ）在儿科呼吸道感染疾病中的感染情况，本研究采用套式ＰＣＲ联合检测儿科呼吸道患儿咽拭子标本中ＭｐｎＤＮＡ和ＭｇＤＮＡ。结果：上呼吸道感染、支气管炎、肺炎、支气管哮喘患儿咽拭子标本中均检出ＭｐｎＤＮＡ和ＭｇＤＮＡ，阳性率分别在２１．９－４０．８％和２１．７－３１．３％。混合感染占总阳性率的１０．０－２８．５％。结论：在儿科呼吸道感染疾病中Ｍｐｎ和Ｍｇ感染率较高，并且存在混合感染，应引起临床医师重视并加强研究 In order to investigate the infection of Mycoplasma pneumoniae (Mpn) and Mycoplasma genitalium (Mpn) in pediatric respiratory infections, we used nested PCR to detect MpnDNA and MgDNA in throat swabs of pediatric respiratory patients. Results: MpnDNA and MgDNA were detected in throat swabs of upper respiratory tract infection, bronchitis, pneumonia and bronchial asthma. The positive rates were 21.9-40.8% and 21.7-31.3% respectively. Mixed infection accounted for 10.0-28.5% of the total positive rate. Conclusions: Mpn and Mg infection rates are higher in pediatric respiratory infections and there is a mixed infection, which should be paid more attention and intensified by clinicians